Efficacy of Ten Structurally Related Essential Oil Components on Anti-biofilm and Anti-quorum Sensing against Fish Spoilers <i>Pseudomonas</i> and <i>Aeromonas</i>

Biofilm formation causes persistent contamination of spoilage microorganisms, leading to serious economic losses in the aquatic processing industry. The antibacterial and anti-biofilm activity ten carvacrol, cinnamaldehyde, derivatives against two fish spoilers, Pseudomonas fluorescens Aeromonas salmonicida, were evaluated by minimum inhibitory concentration (MIC), crystal violet staining, exopolysaccharide, adhesion, swimming, swarming. Ten essential oils (EOs) tested showed different activity, which carvacrol thymol exhibited lowest MIC. Biofilms exopolysaccharide production repressed all EOs at one-fourth MIC without affecting cell viability, as well as, adhesion. Swimming swarming strains significantly decreased. Carvacrol eugenol exerted best P. (42.2%) A. salmonicida (62.9%), respectively. All components that decrease acyl-homoserine lactone (AHL) quorum sensing (QS) signals positively correlated with based on violacein production. Molecular docking revealed most active EO, competed C4-HSL bind receptor LuxR AsaR its hydroxyl group, resulting repression biofilm formation. These results indicated efficacy antibiofilm for was associated structure compounds, strongest mediated QS strains.